a;-L-Rhamnosidase and b-D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis

ELIADES L. A.; ROJAS, N. L.; CABELLO, M; VOGET, C. E.; SAPARRAT M.C.N.

JOURNAL OF BASIC MICROBIOLOGY

WILEY-V C H VERLAG GMBH

Lugar: Jena; Año: 2011 vol. 51 p. 1 - 7

0233-111X

α-L-Rhamnosidases (EC 3.2.1.40) and β-D-glucosidases (EC 3.2.1.21) obtained from several microbialsources are potential catalysts used in food, beverage, and pharmaceutical industries.However, the enzyme preparations currently used have limitations related to the stabilityand activity of the enzyme as well to their reuse. A microtiter screening was carried out in55 fungal strains isolated from alkaline soils, to obtain active α-L-rhamnosidases and β-D-glucosidasesat pH 9.0. While α-L-rhamnosidase activity was detected in 45% of the strains tested,β-D-glucosidase activity was found only in 27%. Based on the fungal ability to produce α-Lrhamnosidaseactivity, cultures were supplemented with naringin to study the activities of theenzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of thefungal strains tested increased the activities of both enzymes in the naringin-supplementedcultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteoalbusLPSC 427 (15.3 fold) for α-L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996(51.1 fold) for β-D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringinat pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one whichshowed highest hydrolysis. Here, different fungal species are reported for the first time fortheir ability to produce α-L-rhamnosidase and β-D-glucosidase activity at alkaline pH.