Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis

MADANES, DANIELA; MERESMAN, GABRIELA; VALLA, SOFÍA A; HASSAN, NOURHAN; KIESEL, LUDWIG; GREVE, BURKHARD; BARAÑAO, ROSA INÉS; GÖTTE, MARTIN; RICCI, ANALÍA GABRIELA

REPRODUCTIVE BIOMEDICINE ONLINE

REPRODUCTIVE HEALTHCARE LTD

Año: 2022

1472-6483

Research question: Does resveratrol exert a potent inhibitory effect on the development of endometriosis by interfering with some pivotal processes?Design: In vitro cultures of primary endometriotic stromal cells, immortalized endometrial stromal (St-T1b) and endometriotic epithelial (12Z) cells were utilized to assess the effects of resveratrol on endometrial cell mechanisms. The effects of resveratrol on 12Z and St-T1b cell viability were assessed by MTT assay, apoptosis by FITC Annexin V assay and cleaved caspase-3 levels, and cell migration by wound healing assay. RT-qPCR was performed to evaluate the effect of resveratrol on the expression of genes related to cell migration, angiogenesis and cell stemness. Results: Resveratrol significantly decreased cell viability (12Z: p=0.0180 and p