Evaluation of sugarcane bagasse and flower stems as substrates for cellulase production by Bacillus amyloliquefaciens C18 A

HEREDIA JUAN PABLO; SÁNCHEZ ETNA MILENA

RESEARCH JOURNAL OF BIOTECHNOLOGY

RESEARCH JOURNAL BIOTECHNOLOGY

Año: 2021

0973-6263

Abstract ? Microbial cellulase production largely depends on the substrates and microorganisms used. Some lignocellulosic agroindustrial wastes can be utilized to produce these enzymes, thus avoiding environmental pollution. This study evaluated the production of cellulases by Bacillus amyloliquefaciens (isolate C18A) in culture media prepared from sugarcane bagasse and flower stem waste; the media contained 56.97% w/w and 58.54% w/w cellulose, respectively. The media was incubated for 72 hours at 37 °C, and samples were taken every 12 hours to evaluate the cellulose degradation and enzymatic activity in the crude extracts at pH 5.0 and pH 7.0. As positive control cellulases were produced in Mandels media using same conditions of incubation and microorganism. The cellulolytic activity at pH 7.0 was higher (35.06 U/mL) for the cellulases produced in the flower stem medium, while the enzymatic activity at pH 5.0 was higher (39.09 U/mL) for the cellulases obtained from the bagasse medium. Cellulases produced in Mandels medium presented activities of 29.94 ± 0.05 U/mL at pH 7.0 and 31.72 ± 0.06 U/mL at pH 5.0, demonstrating that these wastes are sources of available cellulose that can be utilized for cellulase production by B. amyloliquefaciens C18A.