Selenium stress response of the fruit origin strain Fructobacillus tropaeoli CRL 2034

MARTÍNEZ, FERNANDO GABRIEL; GUSTAVO MORENO MARTIN; FERNANDA MOZZI; YOLANDA MADRID; MICAELA PESCUMA

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

SPRINGER

Lugar: Berlin; Año: 2023

0175-7598

Fructobacillustropaeoli CRL 2034 can biotransform selenium into seleno-nanoparticlesand selenocysteine. The proteomic analysis of F. tropaeoli CRL 2034exposed to 5 and 100 ppm of Se showed a dose-dependent response (19 and 77proteins were deregulated, respectively). The deregulated proteins when 5 ppmof Se were used belonged mainly to the categories of energy productionand conversion or had unknown functions, while when cells were grown with 100ppm of Se most of the proteins were grouped into amino acid transport andmetabolism, nucleotide transport and metabolism, or into unknown functions.However, under both Se conditions glutathione reductases were overexpressed(1.8- 3.1), while mannitol 2-dehydrogenase was downregulated (0.54- 0.19).Mannitol 2-dehydrogenase was the only enzyme found to contain SeCys and itsactivity was increased after 5 ppm of Se exposure (1.27 times). The selenizedcells of this strain showed increased resistance to subsequent oxidativestress.