CIHIDECAR 12529
CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Unidad Ejecutora - UE
artículos
Título:
Sample preparation for sequencing hits from one-bead-one-peptide combinatorial libraries by MALDI-TOF-MS
Autor/es:
MARÍA C. MARTÍNEZ CERÓN, SILVANA L. GIUDICESSI, MARIELA M. MARANI, FERNANDO ALBERICIO, OSVALDO CASCONE, ROSA ERRA-BALSELLS, SILVIA A. CAMPERI
Revista:
ANALYTICAL BIOCHEMISTRY
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2010 vol. 400 p. 295 - 297
ISSN:
0003-2697
Resumen:
Different strategies for the identification of peptides selected after the screening of one-bead-one-peptide libraries were assessed, involving the fine tuning of the whole process. Guanidine, widely utilised for bead wash, was replaced by MeCN/AcOH/H2O, this improving matrix crystallisation. Peptide-bead cleavage with NH4OH was cheaper, safer and as efficient as NH3/THF. Peptide elution in micro tubes instead of placing the beads in the sample plate yielded more sample aliquots. Successive-dry-layers deposit sample preparation was better than dried-droplet method. Among the matrices analysed, a-cyano-4-hydroxycinnamic acid resulted in the best peptide ion yield. Cluster formation was minimised by additives addition to the matrix.
