CIHIDECAR 12529
CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Unidad Ejecutora - UE
artículos
Título:
Fluorescent Ratiometric MFC Probe Sensitive to Early Stages of α-Synuclein Aggregation
Autor/es:
DMYTRO A. YUSHCHENKO, JONATHAN A. FAUERBACH, SHYAMALA THIRUNAVUKKUARASU, ELIZABETH A. JARES-ERIJMAN AND THOMAS M. JOVIN
Revista:
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2010 vol. 132 p. 7860 - 7861
ISSN:
0002-7863
Resumen:
We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease.
