Evaluation of Myo-inositol Effects upon Frozen-Thawed Bovine Sperm Quality

VERÓN, GUSTAVO LUIS; PALADEA, ROCÍO; BELLO, RICARDO; MANJON, ANIA ANTONELLA; MONTI, JUAN IGNACIO; VAZQUEZ-LEVIN, MÓNICA HEBE

International Journal of Animal Breeding and Genetics

International Scholars Journals

Año: 2021 vol. 10 p. 1 - 15

Despite great advances in standardization of bovine sperm cryopreservation, frozen-thawed sperm quality is commonly suboptimal. Semen supplementation with various compounds has been addressed to overcome the negative impact of cryopreservation upon sperm quality. While a mild enhancement in human semen parameters was reported after Myo-inositol (MI) supplementation, information is still scarce. This work aimed to evaluate the impact of MI supplementation (2mg/mL) upon sperm quality in frozen-thawed bovine sperm from 9 bulls (breeds: 5 dairy, 4 beef). Evaluations included assessment of total/progressively motile sperm and kinematic parameters (ComputerAssisted Sperm Analysis), sperm vitality, membrane osmotic competence, acrosomal status, chromatin compaction and DNA fragmentation. As a result, an increase (P60% improvement in progressive motility, and 6/9>30% improvement. MI supplementation did not alter sperm vitality, membrane osmotic competence, acrosome status, chromatin compaction and DNA fragmentation. In summary, MI supplementation of frozen-thawed bovine spermatozoa led to a significant improvement in flagellum movement, as evidenced by the increase in sperm kinematics values.