Self-assembly Dynamics and effect on synthetic Nano-Bio-Optical properties by Silica Nanoparticle labelling of Escherichia Coli bacteria

LUNA R. GOMEZ-PALACIOS; CARINA SALINAS; ALICIA V. VEGLIA; VALERIA AME; A. GUILLERMO BRACAMONTE; A. G. BRACAMONTE*

JOURNAL OF NANOPHOTONICS

SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS

Año: 2022 vol. 16 p. 1 - 19

1934-2608

In the present Research work it was studied the deposition of Silica Nanoparticles on Escherichia Coli bacteria. Based on non-covalent interaction between the silanized surfaces and polar components of Biomembrane was achieved the respective Nano-Biostructure. This hybrid architecture showed well stable conformation properties evaluated by different Microscopy techniques such as Bright Field Confocal Microscopy and Transmission Electron Microscopy (TEM). By this manner, the Nano-Bio-architectures were detected within colloidal dispersions as well as in absence of aqueous media. So, the Nano-Bio-interactions were related with the strongest permitted by polar and hydrogen bridges. By this manner it was observed by Bright Field and TEM well constituted and defines Nano-Biostructures after their preparation in optimal conditions. However, in order to evaluate their stabilities and inter-Nano-Biostructure interactions it was determined size distributions within variable period of times. Thus, it was recorded variable Nano-Bio-aggregate sizes depending of Nanoparticles and Bacteria concentrations. It was observed from single Nano labelled Escherichia Coli bacteria with well dispersible properties al low bacteria concentrations. While intermediate and higher concentrations it was observed different distributions of Nano-Biostructures in different period of times. Therefore it was observed that the incorporation of Silica Nanoparticles on Escherichia Coli augmented their dispersibility; however based on their modified membranes with silanized Nano-surfaces increased their inter-Nano-Biostructure interactions with the pass of the time. In this way, it was discussed evaluated its dynamics, and Nano-Bio-Optics properties as well. In this perspective was highlighted that the Nano-Biostructures could not being considered as static systems; so at the place it should be considered their interactions as important factors for dispersibility, stability, and effects against to additional chemical agents such as antibiotics.