INVESTIGADORES
ETCHENIQUE Roberto Argentino
artículos
Título:
Modulating Surface Density of Proteins via Caged Surfaces and Controlled Light Exposure
Autor/es:
ALVAREZ, MARTA; ALONSO, JOSE MARIA; FILEVICH, OSCAR; BHAGAWATI, MANIRAJ; ETCHENIQUE, ROBERTO; PIEHLER, JACOB; DEL CAMPO, ARANZAZU
Revista:
LANGMUIR
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington DC; Año: 2011 vol. 27 p. 2789 - 2795
ISSN:
0743-7463
Resumen:
We demonstrate the possibility of tuning the degree of
functionalization of a surface using photoactivatable chemistries and
controlled light exposure. A photosensitive organosilane with a
protected amine,terminal group and a tetraethyleneglycol spacer was
synthesized. A o-nitrobenzyl cage was used as the photoremovable group
to cage the amine functionality. Surfaces with phototunable amine
densities were generated by controlled irradiation of silica substrates
modified with the photosensitive anchor. Protein layers with different
densities could be obtained by successive coupling and assembly steps.
Protein surface concentrations were quantified by reflectance
interference. Our results demonstrate that the protein density
correlates with the photogenerated ligand density. The density control
was proved over four coupling steps (biotin, SAv, (BT)tris-NTA, MBP, or
GFP), indicating that the interactions between underlying layer and
soluble targets are highly specific and the immobilized targets at the
four levels maintain their full functionality. Protein micropattems
with a gradient of protein density were also obtained.