The role of neuropeptides in regulating ecdysis and reproduction in the hemimetabolous insect Rhodnius prolixus

STERKEL, MARCOS; VOLONTÉ, MARIANO; ALBORNOZ MAXIMILIANO ; WULFF, JUAN PEDRO; DEL HUERTO SANCHEZ MARIANA; PAULA MARÍA TERÁN; AJMAT, MARÍA TERESA; ONS SHEILA

JOURNAL OF EXPERIMENTAL BIOLOGY

COMPANY OF BIOLOGISTS LTD

Lugar: Cambridge; Año: 2022

0022-0949

In ecdysozoan animals, moulting entails the production of a new exoskeleton and shedding the old one during ecdysis. It is induced by a pulse of ecdysone that regulates the expression of different hormonal receptors and activates a peptide-mediated signalling cascade. In Holometabola, the peptidergic cascade regulating ecdysis has been well described. However, very little functional information regarding the neuroendocrine regulation of ecdysis is available for Hemimetabola, which displays an incomplete metamorphosis. We use Rhodnius prolixus as a convenient experimental model to test two hypotheses: (a) the role of neuropeptides that regulate ecdysis in Holometabola is conserved in hemimetabolous insects; (b) the neuropeptides regulating ecdysis play a role in the regulation of female reproduction during the adult stage. The RNA interference-mediated reduction of ETH expression in fourth-instar nymphs resulted in lethality at the expected time of ecdysis. Unlike in holometabolous insects, the knockdown of ETH and OKA did not affect oviposition in adult females, pointing to a different endocrine regulation of ovary maturation. However, ETH knockdown prevented egg hatching. The blockage of egg hatching appears to be a consequence of embryonic ecdysis failure. Most of the first-instar nymphs hatched from the eggs laid by females injected with dsEH, dsCCAP and dsOKA died at the expected time of ecdysis, indicating the crucial involvement of these genes in post-embryonic development. No phenotypes were observed upon CZ knockdown in nymphs or adult females. The results are relevant for evolutionary entomology and could reveal targets for neuropeptide-based pest control tools.