INVESTIGADORES
RIVAS gustavo adolfo
artículos
Título:
Amperometric biosensor for the quantification of gentisic acid using polyphenol oxidase modified carbon paste electrode
Autor/es:
M. L. PEDANO ; G. A. RIVAS
Revista:
TALANTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2000 vol. 53 p. 485 - 495
ISSN:
0039-9140
Resumen:
The affinity of mushroom polyphenol oxidase (PPO) towards gentisic acid (GA), a metabolite of acetyl salicylic acid (ASA), is demonstrated by spectrophotometry and by electrochemical techniques. The enzyme can selectively recognize GA even in the presence of large excess of ASA and its metabolic derivatives (salicylic acid (SA) and salicyluric acid (SUA)). At 0.150 V, the sensitivity is (6.190.1)¡¡104 NAM1, the response is linear up to 2.0¡¡104 M and the detection limit is 5.0¡¡105 M. The kinetic parameters, obtained from Eadie¨CHofstee plots, are Imax-51.4 nA and Km app-6.7¡¡104 M.
