INVESTIGADORES
BOCCACCIO Graciela Lidia
artículos
Título:
Myelin basic protein mRNA localization and polypeptide targeting.
Autor/es:
BOCCACCIO, G.L.; COLMAN, DR
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Año: 1995 vol. 42 p. 277 - 286
ISSN:
0360-4012
Resumen:
Myelin basic proteins (MBPs), the major peripheral membrane proteins of
central nervous system (CNS) myelin, are encoded by mRNAs that are selectively
segregated to the myelinating processes of oligodendrocytes. In order to test
whether the intracellular mechanisms responsible for MBP mRNA translocation are
oligodendrocyte-specific, or alternatively, are present in other cell types and
may therefore be more general, we have studied the localization of the 14 kD MBP
mRNA and its encoded polypeptide (MBP14) in transiently transfected HeLa cells
(a cervical carcinoma cell line) and in the rat pheochromocytoma cell line PC12.
Unlike the situation in oligodendrocytes in situ, where MBP mRNAs are
translocated and become "centrifugally" distributed, in both of the non-glial
cells MBP mRNA was primarily detected in the perinuclear region. The MBP14
polypeptide was found associated with intracellular membranes, and not
exclusively with the plasma membrane. Our results indicate that the inability of
HeLa and PC12 cells to correctly target MBP mRNAs to the cell periphery leads to
a failure to incorporate MBP polypeptides directly into the plasma membrane.
Further, the data lend credence to the concept that MBP mRNA segregation appears
to be a specific feature of myelin-forming cells which is required for the
precise delivery of the encoded polypeptides to the forming myelin
membrane.