INVESTIGADORES
BOCCACCIO Graciela Lidia
artículos
Título:
Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules
Autor/es:
MV BAEZ; GL BOCCACCIO
Revista:
JOURNAL OF BIOLOGICAL CHEMISTRY
Referencias:
Año: 2005 vol. 280 p. 43131 - 43140
ISSN:
0021-9258
Resumen:
Cytoplasmic events depending on RNA
binding proteins contribute to the fine-tuning
of gene expression. Sterile Alpha Motif (SAM)-
containing RNA binding proteins constitute a
novel family of post-transcriptional regulators
that recognize a specific RNA sequence motif
known as Smaug Recognition Element (SRE).
The Drosophila member of this family,
dSmaug, triggers the translational repression
and deadenylation of maternal mRNAs by
independent mechanisms, and the yeast
homologue Vts1 stimulates degradation of
SRE-containing messengers. Two homologous
genes are present in the mammalian genome.
Here we show that hSmaug 1, encoded in
human chromosome 14, represses the
translation of reporter transcripts carrying
SRE motifs. When expressed in fibroblasts,
hSmaug 1 forms cytoplasmic granules that
contain polyadenylated mRNA and the RNA
binding proteins Staufen, TIAR, TIA-1 and
HuR. Smaug 1 foci are distinct from
degradation foci. The murine protein mSmaug
1 is expressed in the central nervous system
and is abundant in post-synaptic densities, a
subcellular region where translation is tightly
regulated by synaptic stimulation. Biochemical
analysis indicated that mSmaug 1 is present in
synaptoneurosomal 20S particles. These results
suggest a role for mammalian Smaug 1 in RNA
granule formation and translation regulation in
neurons.
dSmaug, triggers the translational repression
and deadenylation of maternal mRNAs by
independent mechanisms, and the yeast
homologue Vts1 stimulates degradation of
SRE-containing messengers. Two homologous
genes are present in the mammalian genome.
Here we show that hSmaug 1, encoded in
human chromosome 14, represses the
translation of reporter transcripts carrying
SRE motifs. When expressed in fibroblasts,
hSmaug 1 forms cytoplasmic granules that
contain polyadenylated mRNA and the RNA
binding proteins Staufen, TIAR, TIA-1 and
HuR. Smaug 1 foci are distinct from
degradation foci. The murine protein mSmaug
1 is expressed in the central nervous system
and is abundant in post-synaptic densities, a
subcellular region where translation is tightly
regulated by synaptic stimulation. Biochemical
analysis indicated that mSmaug 1 is present in
synaptoneurosomal 20S particles. These results
suggest a role for mammalian Smaug 1 in RNA
granule formation and translation regulation in
neurons.
Drosophila member of this family,
dSmaug, triggers the translational repression
and deadenylation of maternal mRNAs by
independent mechanisms, and the yeast
homologue Vts1 stimulates degradation of
SRE-containing messengers. Two homologous
genes are present in the mammalian genome.
Here we show that hSmaug 1, encoded in
human chromosome 14, represses the
translation of reporter transcripts carrying
SRE motifs. When expressed in fibroblasts,
hSmaug 1 forms cytoplasmic granules that
contain polyadenylated mRNA and the RNA
binding proteins Staufen, TIAR, TIA-1 and
HuR. Smaug 1 foci are distinct from
degradation foci. The murine protein mSmaug
1 is expressed in the central nervous system
and is abundant in post-synaptic densities, a
subcellular region where translation is tightly
regulated by synaptic stimulation. Biochemical
analysis indicated that mSmaug 1 is present in
synaptoneurosomal 20S particles. These results
suggest a role for mammalian Smaug 1 in RNA
granule formation and translation regulation in
neurons.