INVESTIGADORES
BOCCACCIO Graciela Lidia
artículos
Título:
Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
Autor/es:
BOCCACCIO, GL; CARMINATTI, H; COLMAN, DR
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Referencias:
Año: 1999 vol. 58 p. 480 - 491
ISSN:
0360-4012
Resumen:
The targeting of polypeptides to restricted cytoplasmic
domains by means of mRNA sorting is a widespread
phenomena utilized by many cell types. In the
central nervous system, in situ hybridization analysis
has shown previously that the mRNAs encoding
several myelin-specific proteins are specifically located
within the myelinating processes of oligodendrocytes.
Here, by means of biochemical and subcellular
fractionation methods, we show that a myelin fraction
is selectively enriched in those mRNAs. The four
major myelin basic protein (MBP) mRNAs that arise
by alternative splicing of exons II and VI of the MBP
gene are concentrated in this subcellular fraction.
Furthermore, an interaction of MBP and MOBP 81A
mRNAs with the cytoskeleton was observed. This
interaction might serve to mediate the anchoring of
these messengers after translocation to the subcellular
site of translation.