INVESTIGADORES
RAMIREZ Dario
artículos
Título:
Immuno-spin trapping from Biochemistry to Medicine: advances, challenges and pitfalls. Focus in protein-centered radicals
Autor/es:
GOMEZ-MEJIBA, SE; ZILI ZHAI; SAUTABH CHATTERJEE; RHEAL A. TOWNER; KENNETH HENSLEY; ROBERT FLOYD; MASON, RP; DARIO C. RAMIREZ
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 p. 722 - 729
ISSN:
0304-4165
Resumen:
BACKGROUND: Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduct. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug-induced agranulocytosis, hepatotoxicity, cardiotoxicity, andischemia/reperfusion, as well as in models of neurological, metabolic and immunological diseases.SCOPE OF THE REVIEW: To critically evaluate advances, challenges, and pitfalls as well as thescientific opportunities of IST as applied to the study of protein-centered free radicals generated instressed organelles, cells, tissues and animal models of disease and exposure.
