INVESTIGADORES
RAMIREZ Dario
artículos
Título:
Comment on: Kim et al. Deficiency for Costimulatory Receptor 4-1BB Protects Against Obesity-Induced Inflammation and Metabolic Disorders. Diabetes 2011;60:3159-3168.
Autor/es:
SANDRA E. GOMEZ-MEJIBA; DARIO C. RAMIREZ
Revista:
DIABETES
Editorial:
AMER DIABETES ASSOC
Referencias:
Año: 2012 vol. 61 p. 6 - 7
ISSN:
0012-1797
Resumen:
Diabetes. 2012 Oct;61(10):2405-13. Epub 2012 Jun 14.
In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.
Towner RA1, Smith N, Saunders D, Henderson M, Downum K, Lupu F, Silasi-Mansat R, Ramirez DC, Gomez-Mejiba SE, Bonini MG, Ehrenshaft M, Mason RP.
Author information
Abstract
Oxidative
stress plays a major role in diabetes. In vivo levels of membrane-bound
radicals (MBRs) in a streptozotocin-induced diabetic mouse model were
uniquely detected by combining molecular magnetic resonance imaging
(mMRI) and immunotrapping techniques. An anti-DMPO
(5,5-dimethyl-1-pyrroline N-oxide) antibody (Ab) covalently bound to an
albumin (BSA)-Gd (gadolinium)-DTPA (diethylene triamine penta acetic
acid)-biotin MRI contrast agent (anti-DMPO probe), and mMRI, were used
to detect in vivo levels of DMPO-MBR adducts in kidneys, livers, and
lungs of diabetic mice, after DMPO administration. Magnetic resonance
signal intensities, which increase in the presence of a Gd-based
molecular probe, were significantly higher within the livers, kidneys,
and lungs of diabetic animals administered the anti-DMPO probe compared
with controls. Fluorescence images validated the location of the
anti-DMPO probe in excised tissues via conjugation of streptavidin-Cy3,
which targeted the probe biotin moiety, and immunohistochemistry was
used to validate the presence of DMPO adducts in diabetic mouse livers.
This is the first report of noninvasively imaging in vivo levels of MBRs
within any disease model. This method can be specifically applied
toward diabetes models for in vivo assessment of free radical levels,
providing an avenue to more fully understand the role of free radicals
in diabetes.