INVESTIGADORES
RAMIREZ Dario
artículos
Título:
HYPOTHYROIDISM MODIFIES LIPID COMPOSITION OF POLYMORPHONUCLEAR LEUCKOCYTES
Autor/es:
CORIA, M.; CARMONA, Y.; MARRA, C.A.; SANDRA E. GOMEZ-MEJIBA; DARIO C. RAMIREZ; ANZULOVICH, A.C.; MARIA S. GIMENEZ
Revista:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY : INTERNATIONAL JOURNAL OF EXPERIMENTAL CELLULAR PHYSIOLOGY, BIOCHEMISTRY, AND PHARMACOLOGY.
Editorial:
KARGER
Referencias:
Lugar: Basel; Año: 2012 vol. 29 p. 713 - 724
ISSN:
1015-8987
Resumen:
Thyroid hormones are important regulators of lipid metabolism.
Polymorphonuclear leukocytes (PMN) are essential components of innate
immune response. Our goal was to determine whether hypothyroidism
affects lipid metabolism in PMN cells. Wistar rats were made hypothyroid
by administrating 0.1 g/L 6-propyl-2-thiouracil (PTU) in drinking water
during 30 days. Triacylglycerides (TG), cholesterol and phospholipids
were determined in PMN and serum by conventional methods. The mRNA
expression of LDL receptor (LDL-R), 3-hydroxy-3-methylglutaryl-CoA
reductase (HMGCoAR), sterol regulatory element binding protein 2
(SREBP-2), and diacylglycerol acyltransferase 2 (DGAT-2) were quantified
by Real-Time PCR. Cellular neutral lipids were identified by Nile red
staining. We found hypothyroidism decreases serum TG whereas it
increases them in PMN. This result agrees with those observed in Nile
red preparations, however DAGT-2 expression was not modified.
Cholesterol synthesizing enzyme HMGCoAR mRNA and protein was reduced in
PMN of hypothyroid rats. As expected, cholesterol content decreased in
the cells although it increased in serum. Hypothyroidism also reduced
relative contents of palmitic, stearic, and arachidonic acids, whereas
increased the myristic, linoleic acids, and the unsaturation index in
PMN. Thus, hypothyroidism modifies PMN lipid composition. These findings
would emphasize the importance of new research to elucidate
lipid-induced alterations in specific function(s) of PMN.