INVESTIGADORES
CAPANI Francisco
artículos
Título:
EFFECTS OF PERINATAL ASPHYXIA ON RAT STRIATAL CYTOSKELETON
Autor/es:
GE SARACENO; MV AYALA; MS BADORREY; M HOLUBIEC; ROMERO J; GEORGE BARRETO; GIRALDEZ ALVAREZ; KOLLIKER FRERS; COIRINI H.; CAPANI F
Revista:
SYNAPSE
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Año: 2012 p. 9 - 19
ISSN:
0887-4476
Resumen:
Perinatal asphyxia (PA) is a medical condition associated with a high
short-term morbimortality and different long-term neurological diseases. In previous
works, we have shown that neuronal and synaptic changes in rat striatum lead to ubiprotein
accumulation in post-synaptic density (PSD) after six months of sub-severe PA.
However, very little is known about the synaptic and related structural modifications
induced by PA in young rats. In the present work, we studied neuronal cytoskeleton
modifications in striatum induced by subsevere PA in 30-day-old rats. We observed a
significant decrease in the number of neurons, in particular calbindin immunoreactive
neurons after PA. In addition, it was also observed that actin cytoskeleton was highly
modified in the PSD as well as an increment of F-actin staining by Phalloidin-alexa488488
in the striatum of PA rats. Using correlative fluorescence-electron microscopy photooxidation,
we confirmed and extended confocal observations. F-actin staining augmentation
was mostly related with an increment in the number of mushroom-shaped
spines. Consistent with microscopic data, Western blot analysis revealed a b-actin
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.
b-actin
increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was
decreased after PA, being NF-200 expression unmodified. Although neuronal death was
observed, signs of generalized neurodegeneration were absent. Taken together these
results showed early post-synaptic F-actin cytoskeleton changes induced by PA with
slightly modifications in the other components of the neuronal cytoskeleton, suggesting
that F-actin accumulation in the dendritic spines could be involved in the neuronal loss
induced by PA. Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.Synapse 66:9?19, 2012. VVC 2011 Wiley Periodicals, Inc.