INVESTIGADORES
CAPANI Francisco
artículos
Título:
Nitric oxide increases in the rat retina after continuos illumination
Autor/es:
L. PIEHL , F. CAPANI, G. FACORRO , E.M. LÓPEZ, E. RUBIN DE CELIS , C. PUSTOVRH,
Revista:
BRAIN RESEARCH
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2007 p. 112 - 119
ISSN:
0006-8993
Resumen:
Continuous illumination (CI) of the retina induces an oxidative stress followed by the
degeneration of photoreceptors. This phenomenon may be partially related to the excessive
production of nitric oxide (NO). In order to confirm this hypothesis, the aims of this work are
to determine NO levels during the illumination of the retina by electron paramagnetic
resonance (EPR), and if an increase of NO is found, to characterize the NOS isoform
responsible of the increment by using Western blot. SpragueDawley rats were continuously
illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were
maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed
in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the
expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show
significant differences of nNOS among illuminated and control retinas. In summary, there is
an increase of NO during CI. Further studies will reveal whether this mechanism is
responsible for light induced photoreceptor degeneration.
illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were
maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed
in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the
expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show
significant differences of nNOS among illuminated and control retinas. In summary, there is
an increase of NO during CI. Further studies will reveal whether this mechanism is
responsible for light induced photoreceptor degeneration.
illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were
maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed
in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the
expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show
significant differences of nNOS among illuminated and control retinas. In summary, there is
an increase of NO during CI. Further studies will reveal whether this mechanism is
responsible for light induced photoreceptor degeneration.
illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were
maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed
in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the
expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show
significant differences of nNOS among illuminated and control retinas. In summary, there is
an increase of NO during CI. Further studies will reveal whether this mechanism is
responsible for light induced photoreceptor degeneration.
illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were
maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed
in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the
expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show
significant differences of nNOS among illuminated and control retinas. In summary, there is
an increase of NO during CI. Further studies will reveal whether this mechanism is
responsible for light induced photoreceptor degeneration.
Dawley rats were continuously
illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were
maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed
in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the
expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show
significant differences of nNOS among illuminated and control retinas. In summary, there is
an increase of NO during CI. Further studies will reveal whether this mechanism is
responsible for light induced photoreceptor degeneration.