Small-angle X-ray scattering to quantify the incorporation and analyze the disposition of magnetic nanoparticles inside cells

D. F. CORAL; P.A.SOTO; DE SOUSA,M.ELISA; M.E.F. BROLLO; J.AMERA-CÓRDOBA; C.P.SETTON-AVRUJC; A.ROIG; FERNANDEZ VAN RAAP, MARCELA

JOURNAL OF COLLOID AND INTERFACE SCIENCE

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2022

0021-9797

Access to detailed information on cells loaded with nanoparticles with nanoscale precision is of a longstanding interest in many areas of nanomedicine. In this context, designing a single experiment able toprovide statistical mean data from a large number of living unsectioned cells concerning information onthe nanoparticle size and aggregation inside cell endosomes and accurate nanoparticle cell up-take is ofparamount importance. Small-angle X-ray scattering (SAXS) is presented here as a tool to achieve suchrelevant data. Experiments were carried out in cultures of B16F0 murine melanoma and A549 humanlung adenocarcinoma cell lines loaded with various iron oxide nanostructures displaying distinctivestructural characteristics. Five systems of water-dispersible magnetic nanoparticles (MNP) of differentsize, polydispersity and morphology were analyzed, namely, nearly monodisperse MNP with 11 and13 nm mean size coated with meso-2,3-dimercaptosuccinic acid, more polydisperse 6 nm colloids coatedwith citric acid and two nanoflowers (NF) systems of 24 and 27 nm in size resulting from the aggregationJournal of Colloid and Interface Science 608 (2022) 1?12Contents lists available at ScienceDirectJournal of Colloid and Interface Sciencejournal homepage: www.elsevier.com/locate/jcisSynchrotron radiation, soft materA549, B16F0, cell up-takeof 8 nm MNP. Up-take was determined for each system using B16F0 cells. Here we show that SAXS pattern provides high resolution information on nanoparticles disposition inside endosomes of the cytoplasm through the structure factor analysis, on nanoparticles size and dispersity after theirincorporation by the cell and on up-take quantification from the extrapolation of the intensity in absolutescale to null scattering vector. We also report on the cell culture preparation to reach sensitivity for theobservation of MNP inside cell endosomes using high brightness SAXS synchrotron source. Our resultsshow that SAXS can become a valuable tool for analyzing MNP in cells and tissues.