Multiphoton single-molecule localization by sequential excitation with light minima

MASULLO, LUCIANO A.; STEFANI, FERNANDO D.

Light: Science & Applications

Springer Nature Limited

Lugar: London; Año: 2022 vol. 11

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1?2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.