-A one-week laboratory practice: Introducing the students to the study of plant biochemistry and signal transduction

ULLOA RITA; RAICES M; TÉLLEZ-IÑÓN MT

Bioscene

Association of College and University Biology Educators

Año: 2000 vol. 26 p. 3 - 12

This report describes a one-week laboratory practice for students. An approach to study a Calcium dependent protein kinase (CDPK) involved in signal transduction processes in potato plants, is undertaken. A number of basic biochemical techniques including the partial purification of a protein kinase, protein kinase activity assays, protein determination, SDS-PAGE analysis of phosphorylated proteins and Western blot assays are described.