Análisis ontogénico de células mucosas intestinales en juveniles diploides y triploides de Rhamdia quelen

MUÑOZ, L.; LÓPEZ, P.A.; MASCAMBRONI, M.B.; MORÓN-ALCAIN, E.; VIGLIANO, F.A.

Corrientes

Simposio; V Simposio Argentino de Ictiología; 2017

Instituto de Ictiología del Nordeste

La triploidía e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> una herramienta utilizada para generar e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>terilidad gamética. A nivel ti<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ular, produce hipertrofia que conlleva cambio<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>iológico<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>. La<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> célula<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> muco<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>a<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> (CM<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>) pre<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ente<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en el epitelio inte<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tinal cumplen funcione<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de lubricación y protección fí<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ica, inmunológica<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y de participación en lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> proce<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>o<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> dige<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tivo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> a travé<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de la <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ecreción de mucina<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>.Por ello, no<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> propu<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>imo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> comparar el área y den<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>idad de CM<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en inte<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tino a<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>cendente (IA), de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>cendente (ID) y convoluto (IC) de R. quelen diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> (GC) y triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> obtenido<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> por inmer<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ión de huevo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fecundado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en agua a 4ºC (TF) o a 36ºC (TC). Se mue<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>trearon 10 ejemplare<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en cada grupo entre lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> 5 y 148 día<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> po<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>t-eclo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ión (dpe). En corte<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> hi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tológico<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> teñido<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> con PAS, <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e midieron amba<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> variable<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y la<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> diferencia<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e analizaron con te<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>t de ANOVA a un criterio (po<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>te<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>t Tukey).La den<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>idad de CM<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> no pre<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>entó diferencia<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativa<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> entre grupo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> para un mi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>mo día de mue<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>treo. Por otra parte, <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e ob<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ervó un incremento <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativo en e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ta variable de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>de lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> 48 dpe en IA e ID en todo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> grupo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>. El área de CM<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fue <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativamente <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>uperior en lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> TC en IA e ID re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto a lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> demá<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> grupo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>de lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> 33 dpe en adelante. Dentro de cada grupo, <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e ob<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ervó un incremento paulatino en la variable con diferencia<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativa<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> que coincidieron con el <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>umini<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tro exclu<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ivo de ración balanceada <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>eca.La hipertrofia de CM<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en TC puede relacionar<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e con un po<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ible incremento en la demanda de inge<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ta a<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ociada al mayor crecimiento de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>crito en triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de R. quelen. Sin embargo, la au<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>encia de diferencia<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativa<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> entre TF y GC <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ugiere que lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> efecto<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> ti<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ulare<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de la triploidización no <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ólo dependen del nivel de ploidía <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>i no también del método empleado para la obtención de lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> ejemplare<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>.