Evaluación del desarrollo en larvas diploides y triploides de Rhamdia quelen sometidas a un régimen de ayuno-realimentación

BOAGLIO, A.C.; RUBINO, O.; MENDÍA, A.C.; MUÑOZ, L.; MORÓN-ALCAIN, E.; MASCAMBRONI, M.B.; ARRANZ, S.E.; VIGLIANO, F.A.

Rosario

Congreso; XX Congreso y XXXVIII Reunión Anual de la Sociedad de Biología de Rosario; 2018

Sociedad de Biología de Rosario

La triploidización y manejo nutricional <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e u<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>an como herramienta<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> tanto para mejora productiva como para evaluar mecani<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>mo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>iológico<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> relacionado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> al crecimiento y de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>arrollo de pece<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en cultivo. La aplicación de e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ta<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> herramienta<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> induce cambio<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> ligado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> al crecimiento <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>omático tanto a nivel ti<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ular como en la expre<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ión de molécula<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> reguladora<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> del de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>arrollo mu<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>cular. En ba<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e a e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>to, evaluamo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> cambio<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tructurale<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y moleculare<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en mú<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>culo blanco de larva<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de R. quelen bajo di<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tinta<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> condicione<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> nutricionale<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>. Se u<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>aron huevo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fecundado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de R. quelen proveniente<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de una mi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ma reproducción artificial. La mitad de la de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ova <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>umergió en agua a 36°C 5 min para la obtención de triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>. A 28 día<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> po<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>t-eclo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ión <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e ayunaron larva<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> por 30 hora<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>, con<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tituyéndo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e grupo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> (DA) y triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> (TA) ayunado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>. Luego, diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> ayunado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fueron realimentado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>acrificado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> pa<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ada<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> 1 y 3 hora<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>, conformando lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> grupo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> DAR-1, DAR-3, TAR-1 y TAR-3. Se u<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>aron controle<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de ayuno diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> (DC) y triploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> (TC) alimentado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>in re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>triccione<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>. En todo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> grupo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e extrajeron mue<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tra<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de mú<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>culo blanco para determinar variable<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> morfométrica<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en corte<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> hi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tológico<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> y nivele<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de expre<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ión proteica relativa de miogenina y mio<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tatina por We<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tern Blot. Se ob<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ervó aumento <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativo en el Nº de Fibra<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> mu<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>culare<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>/mm2 de DA (4326±181) re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto de DC (2878±130) y TA (3650±103). Por otra parte, TAR-1 (3341 ±164) regi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tró un aumento en e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ta variable re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto de DAR-1 (2657±81). Ademá<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>, el % fibra<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> < 500 μm2 en DA (92±1 %) evidenció un aumento re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto de DC (79±2 %) y DAR-1(75±2 %). Por otra parte, DAR-1 pre<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>entó una di<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>minución re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto de TAR1(84±1 %). También <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e regi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tró una diferencia <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ignificativa entre DC y DAR-3 (3420±111). Re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto al área tran<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ver<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>al de la<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> fibra<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>, DA (220±10 μm2) mo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tró una di<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>minución comparado con DC (293±11 μm2). A <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>u vez, <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e regi<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tró incremento de e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ta variable en DAR-1 (352±10 μm2) re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto de DC y TAR-1. También <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e detectaron diferencia<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en la expre<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ión de miogenina <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ólo en DA (1,3±0,07) re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecto de DC (1,06±0,01), a<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>í como entre é<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>te grupo y TC (0,7±0,02). Lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ultado<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> morfométrico<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e analizaron con ANOVA a un criterio (po<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>te<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>t Sidak), mientra<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> que lo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de We<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tern Blot <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e analizaron con t-te<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>t. Concluimo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> que la re<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tricción alimentaria impacta en mayor medida <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>obre la morfología y diferenciación de célula<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> mu<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>culare<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> de larva<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> diploide<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>, manife<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>tándo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>e po<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>ible<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> diferencia<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> en la utilización de la energía, regulación del metaboli<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>mo y mantenimiento mu<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>cular. E<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>to<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> hallazgo<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> aportan nuevo conocimiento <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>obre el de<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>arrollo de R. quelen y podría <<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>er de interé<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan> para el manejo del cultivo de la e<<span class="keyword">sspan>pan cla<span class="keyword">sspan><span class="keyword">sspan>="keyword"><span class="keyword">sspan>span class="keyword">sspan>pan>pecie.