Characterization of leukocytes in the blood of Senegalese sole (Solea senegalensis).

FAÍLDE, D.; VIGLIANO, F.A.; LOSADA, A.P.; BERMÚDEZ, R.; VÁZQUEZ, M.; QUIROGA, M.I.

Rosario, Argentina

Congreso; I Congreso Argentino de Ciencias Morfológicas Veterinarias; 2009

Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario.

Senegalese sole (Solea senegalensis) is a fish whose intensive cultivation is in its experimental phase in Spain. The results achieved so far are optimistic, but the knowledge of the physiological parameters of this species, especially blood, is essential to count on basic tools applicable to the diagnosis of diseases. The immunocytochemical methods are poorly developed in teleosts due to the shortage of trade mark antibodies specific to the various cell types. For this reason, the detection of enzymes present in leukocytes is one of the methods of choice in fish haematology. The aim of this study was to quantify and describe by enzyme cytochemistry Senegalese sole leukocytes from peripheral blood. Thrombocytes (59%), lymphocytes (24%), granulocytes (14%) and monocytes (3%) were differentiated. All leukocytes showed a positive reaction to acid phosphatase and specific esterase, while granulocytes and monocytes were positive to Sudan Black B and non-specific esterase, respectively. The thrombocytes presented a relatively large nucleus surrounded by a pale blue cytoplasm. Lymphocytes were characterized by a large indented nucleus and a high nucleus:cytoplasm ratio. The granulocytes displayed a spherical morphology, showing an eccentric nucleus, sometimes lobed, and cytoplasmic granules. Finally, monocytes were the leukocytes with the largest size and presented many variable forms.