INVESTIGADORES
PERULLINI Ana Mercedes
artículos
Título:
Optimizing silica encapsulation of living cells: in situ evaluation of cellular stress
Autor/es:
MERCEDES PERULLINI; MATÍAS JOBBÁGY; MARIANA BERMÚDEZ MORETTI; SUSANA CORREA GARCÍA; SARA A. BILMES
Revista:
CHEMISTRY OF MATERIALS
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2008 p. 3015 - 3021
ISSN:
0897-4756
Resumen:
The encapsulation of living cells in materials with good optical and mechanical properties often produces
death or stress due to the release of toxic byproducts originated during the synthesis. We present here a
method to assess the cellular stress that silica entrapment exerts over living cells taking into account the
main preparation variables such as the nature of the silica source, protecting functional groups, total
solid concentration, or indirect procedures. Measurement of the cellular stress status of genetically modified
Sacharomyces cereVisiae, a true biological probe, allowed us to perform a quantitative analysis of cellular
stress in a short time basis (compared to conventional long-term viability tests), opening the gate for a
more sophisticated approach to optimize the synthesis conditions. In addition, the aforementioned findings
allowed the preparation of novel materials with enhanced optical and mechanical properties. The relation
of cellular stress with initial viability is also discussed.Visiae, a true biological probe, allowed us to perform a quantitative analysis of cellular
stress in a short time basis (compared to conventional long-term viability tests), opening the gate for a
more sophisticated approach to optimize the synthesis conditions. In addition, the aforementioned findings
allowed the preparation of novel materials with enhanced optical and mechanical properties. The relation
of cellular stress with initial viability is also discussed.