INVESTIGADORES
PERULLINI Ana Mercedes
artículos
Título:
Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
Autor/es:
Y. FERRO; M. PERULLINI; M. JOBBÁGY; S. A. BILMES; C. DURRIEU
Revista:
SENSORS
Editorial:
MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI
Referencias:
Lugar: Basel; Año: 2012 vol. 12 p. 16879 - 16891
ISSN:
1424-8220
Resumen:
A new biosensor was designed for the assessment of aquatic environment
quality. Three microalgae were used as toxicity bioindicators: Chlorella
vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas
reinhardtii. These microalgae were immobilized in alginate and silica
hydrogels in a two step procedure. After studying the growth rate of
entrapped cells, chlorophyll fluorescence was measured after exposure to
(3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various
concentrations of the common herbicide atrazine. Microalgae are very
sensitive to herbicides and detection of fluorescence enhancement with
very good efficiency was realized. The best detection limit was 0.1 µM,
obtained with the strain C. reinhardtii after 40 minutes of exposure.