Ultradeformable archaeosomes as new topical adjuvants

HIGA LETICIA H., SCHILRREFF PRISCILA, PEREZ ANA PAULA, IRIRARTE MAIARA A., RONCAGLIA DIANA I., MORILLA MARIA JOSE, ROMERO EDER L.

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 8 p. 1319 - 1328

1549-9634

Ultradeformable archaeosomes (UDA) are vesicles made of soybean phosphatidylcholine (SPC), sodium cholate (NaChol) and polar lipids from Halorubrum tebenquichense (3:1:3 wt/wt). While ultradeformable liposomes (UDL, made of SPC and NaChol at 6:1 wt/wt) and UDA were neither captured nor caused cytotoxicity on keratinocytes, UDA was avidly captured by macrophages, being their viability reduced by 0.4-1.6mg/ml phospholipids, to 60-25 %. Instead, UDL were poorly captured and caused no toxicity. Balb/C mice immunized by the topical route with four doses of ovalbumin (OVA)-loaded UDA, at 75 ?Ýg OVA/600 ?Ýg phospholipids (125 nm mean size and -42 mV Z potential), induced IgG titers ten to one hundred folds higher than those immunized with OVA loaded-UDL at the same dosage. Both matrices penetrate to the same skin depth (nearly 10 ?Ým after 1h on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content. Halorubrum tebenquichense (3:1:3 wt/wt). While ultradeformable liposomes (UDL, made of SPC and NaChol at 6:1 wt/wt) and UDA were neither captured nor caused cytotoxicity on keratinocytes, UDA was avidly captured by macrophages, being their viability reduced by 0.4-1.6mg/ml phospholipids, to 60-25 %. Instead, UDL were poorly captured and caused no toxicity. Balb/C mice immunized by the topical route with four doses of ovalbumin (OVA)-loaded UDA, at 75 ?Ýg OVA/600 ?Ýg phospholipids (125 nm mean size and -42 mV Z potential), induced IgG titers ten to one hundred folds higher than those immunized with OVA loaded-UDL at the same dosage. Both matrices penetrate to the same skin depth (nearly 10 ?Ým after 1h on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content. ?Ýg OVA/600 ?Ýg phospholipids (125 nm mean size and -42 mV Z potential), induced IgG titers ten to one hundred folds higher than those immunized with OVA loaded-UDL at the same dosage. Both matrices penetrate to the same skin depth (nearly 10 ?Ým after 1h on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content. ?Ým after 1h on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content.