INVESTIGADORES
FRANCO paula Gabriela
congresos y reuniones científicas
Título:
OLIGODENDROCYTE-ENRICHED PRIMARY CULTURESFROM MURINE SVZ NEURAL STEM AND PROGENITORSCELLS
Autor/es:
PASQUINI J; SILVESTROFF L; FRANCO P
Lugar:
Long Beach, California
Reunión:
Congreso; 45th ASN Annual Meeting; 2014
Institución organizadora:
ASN
Resumen:
Neural stem and progenitor cells (NSC/NPC) are multipotentand have gained well-earned fame in the past decade, since theirpresence in the adult mammalian brain has made them one ofmany possible targets for CNS disease treatment. We are inter-ested in harnessing NSC fate specification in vitro as a strategyfor neurodegenerative disease cell therapies and cellreplacement therapies. We are currently studying ways toimprove oligodendrogenesis in vitro from neonatal micesubventricular zone primary cultures. The use of PlateletDerived Growth Factor (PDGF), together with EpidermalGrowth Factor (EGF) and basic Fibroblast Growth Factor (bFGF)is well known to favor the fate choice of multipotent neuralcells towards the oligodendroglial lineage. We compareddifferent growth factor and growth factor isoform combinationsin vitro to generate cultures with a suitable biomass and highlyenriched in oligodendrocyte progenitor cells (OPC). The PDGFhomodimeric isoforms AA and BB showed differential effects atequal concentrations on OPC proportions after treatment. Thecombination of both these isoforms together with EGF, bFGF orEGF/bFGF was substantially different. Although biomass wascompromised, the best culture condition for OPC generation inthis culture system was obtained by combining bFGF and PDGF-BB. These results are intended to improve NSC/NPC culturetechniques when OPC-enriched primary cultures are desired.