Pro differentiation effects of gold nanoparticles in primary cultures of neural stem cells

BYRNE A.; MOLINARI Y; PEREZ MJ; GLISONI R; LÁZARO MARTINEZ J; FRANCO, P

Ciudad Autonoma De Buenos Aires

Encuentro; SAN 2022 Meeting; 2022

Sociedad Argentina de Investigación en Neurociencias

In the last few years, gold nanoparticles (GNP) were shown to stimulate thedifferentiation of several cell types. As the development of new therapies topromote remyelination is a high priority for Multiple Sclerosis (MS), here we usedneurospheres (NS) cultures to study the effects of GNP on the different mechanismsinvolved in brain repair.Polyethyleneimine-stabilized GNP of 55 nm of hydrodynamic diameter weresynthesized and used to treat NS cultures. We found a 30% reduction in themetabolic activity of cultures by MTT assay. Although NS numbers were not affectedat any dose, we detected a significant reduction in NS diameter at 10 ppm GNP,which was attributed to the downregulation of proliferation observed by BrdUincorporation assay.In addition, we found a significant inhibition of cell migration in response to GNPtreatment and observed some abnormalities in cell adhesion.Finally, NS cultures undergoing cell differentiation and treated with GNP showed amarked increase in the number of mature oligodendrocytes respect to controls.All these results indicate that GNP inhibit NSC/NPC proliferation and promote celldifferentiation towards the oligodendroglial lineage. These findings support the ideathat GNP could be used for the development of new regenerative strategies for theCNS.