Archaeosomes as bacterioruberine vehicles: stability, antioxidant, anti-inflammatory activity

CAIMI, AT; YASYNSKA, O; ROMORO, EL; MORILLA, MJ

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Reactive oxygen species (ROS) are responsible for the development of certain diseases such as cancer and atherosclerosis through the oxidation of DNA, proteins and lipids. Antioxidant compounds decrease the rate of mutagenesis and carcinogenesis by inhibiting oxidative damage to cells. The extreme halophilic archaebacteria produce a unique group of polar (PA) and neutral membrane archaeolipids (NA). PAs are composed of saturated isoprenoid chains linked by ether bonds to the glycerol carbons in the sn 2,3 position and are highly resistant to hydrolytic, oxidative and enzymatic attack. NA are mainly composed of bacterioruberin (BR), a carotenoid with high antioxidant activity, able to reduce the rate of mutagenesis and carcinogenesis by inhibiting oxidative damage to cells. Its hydrophobicity and susceptibility to oxidation, however, makes its application difficult. The encapsulation of BR in nanovesicles could protect its antioxidant activity. In this work, we prepared archaeosomes made of PA and BR (ARQ-BR) and studied its antioxidant activity compared with the one of nanovesicles made of soybean phosphatidylcholine and BR (LIPO-BR).LIPO-BR (164 ± 10 nm, -16 ± 2 mV Z-potential and 1.3 µg/mg phospholipids/BR) showed a higher IC50% (concentration of BR which scavenge 50% free radicals of DPPH) than ARQ-BR (222 ± 53 nm, -36 ± 3 mV Z-potential and 4.7 µg/mg phospholipids/BR rate): 0.043 mg/ml against 0.082 mg/ml. However, the antioxidant activity of ARQ-BR remained unchanged after incubation at high temperatures (30? at 80°C) while it was completely lost in LIPO-BR. ARQ was able to protect the antioxidant activity of BR, even in hostile conditions, allowing future application and enabling its antioxidant and anti-inflammatory activity