GUT MICROBIOTA ALTERATION IN DIABETIC MICE EXPOSED TO CHRONIC STRESS.

RUBINSTEIN, ROXANA; WALD, MIRIAM; GENARO, ANA M.

Virtual

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC, AAFE, SAI, Nanomed

Gut microbiota is the group of microorganism (commensal, symbiotic and pathogenic) that we find in our gut. It participates in multiple functions and an association between unbalanced microbiota and several diseases, including diabetes, has been reported. Type 1 diabetes (T1D) is characterized by impaired insulin secretion and it has been recognized the contribution of psychosocial factors in T1D. The aim of the present study is to characterize microbiota alterations in diabetic mice exposed to chronic stress. To induced diabetes, we treated male BALB/c mice with multiple low doses of streptozotocin (stz) and then, the animals were subject to chronic mild stress (CMS) by a daily application of different mild stressors. Fecal samples were collected and genomic DNA was extracted. 16s total bacteria, 16s Bacteriodetes and 16s Firmicultes (most abundant component of the microbiota) were measured by qPCR using specific primers. After 13 weeks of CMS S, glycemic levels in diabetic and diabetic + CMS were elevated (p