TLR4 KO MICE ARE LESS SENSITIVE TO THE EFFECTS OF A HIGH-FAT DIET INTAKE THAN C57BL/6J MICE BUT MORE SUSCEPTIBLE TO STRESS

QUIROGA, SOFÍA; MARCONE, PAULA; MANSILLA RICATTI, JULIETA; BURGUEÑO, ADRIANA; GENARO, ANA MARÍA

Virtual

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC, AAFE, SAI, Nanomed

Previously, we and others described that male C57BL/6J (WT) mice are sensitive to diet-induced obesity, leading to an increased inflammatory state. Mice under chronic stress (CS) gained less body weight (BW). TLR4 receptor knockout mice (KO) do not show inflammation under high-fat diet (HFD) intake, although their stress response is unknown. Here we studied the metabolic and behavioral effects of HFD intake and CS exposure in both strains. At 4 weeks of age, mice received a standard diet (SD) or a HFD. After 12 weeks, they were exposed (or not) to CS for 8 weeks. Resulting in 4 groups for each strain: 1) SD, 2) SD+CS, 3) HFD and 4) HFD+CS. We recorded BW, blood glucose (GLU), lipid metabolism and behavioral tests. After 12 weeks of HFD, WT showed higher BW gain (p