PARTICIPATION OF GUT MICROBIOTA ON THE METABOLIC CHANGES INDUCED BY HIGH FAT DIET

MARÍA PAULA MARCONE; MARA ROXANA RUBINSTEIN GUICHÓN; ESTHER N GEREZ; ANDRÉS PROCHNIK; ANA MARIA GENARO; ADRIANA LAURA BURGUEÑO; MIRIAM RUTH WALD

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

SAIC, SAFE, SAB, SAP, NANOMED, AACyTAL

Environmental factors, such as a fat enriched diet are among the causes of the great prevalence of obesity and type 2 diabetes in the population. Recent studies have shown that diet-induced alterations the gut microbiota composition play a pivotal role in the development of obesity. Changes in the predominant gut bacterial phylums: Bacteroidetes and Firmicutes (B-F) characterizes different metabolic phenotypes. Our objective is to study in a high fat diet (HFD) feeding model of obesity if the treatment with probiotics induces changes in glycidic metabolism and B-F DNA. Four weekold male C57B6/6J mice were fed with a normal chow diet (fat content: 7.5 g/100 g) or an HFD diet (fat content: 31 g/100 g, butter and lard). When HFD mice reached a 5 % weight gain with respect to the controls (p= 0.07, n= 12) and a cumulative increase in food intake of 19 % kcal (week 16), probiotic treatment was started. We used two type of probiotics (P1 and P2) in two concentrations: 107 CFU and 108 CFU, supplied in drinking water. Genomic DNA of gut microbiota was isolated from feces samples and were analyzed by real time PCR reactions using selective primers. HFD induced an increment in basal (Gb) and after 2 hours of glucose administration (G2h) glycemia. Lower dose probiotic treatment did not produce changes in those parameters, however the higher dose induced an improvement in Gb and G2h (ANOVA Gb p= 0.0204, G2h p= 0.022, n= 4) being P2 most effective. No changes were observed in body weight and food intake. Concerning gut microbiota, we observed a nonsignificant increase in the Bacteroidetes DNA under treatment with HFD and P1 (interaction diet probiotic p= 0.06, n= 4). We conclude that probiotic treatment improved metabolic parameters that were altered during HFD treatment. These data suggest the importance of gut microbiota as a therapeutic target in the treatment of obesity complications.