Hypothalamic CB1 receptor activation inhibits the inflammatory response induced by endotoxic stress in detrimental of the reproductive function

SURKIN PN; GALLINO SL; DI ROSSO ME; ASTRAUSKAS JI,; GENARO AM; DE LAURENTIS A; FERNÁNDEZ-SOLARI J

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reunion Anual de la Sociedad de Inmunología (SAI), XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE),VII Reunión Anual de la Sociedad Argenti; 2016

SAIC, SAFE, SAI

During neurodevelopment, mice have an increased susceptibilityto the deleterious effects of prenatal time insults, 􀁙hich havean impact on memory, learning and anxiety􀀏related behaviors.Some of these changes are associated 􀁙ith an alteration of theH􀀲A axis response. 􀀹e aim to evaluate the effect of enrichedenvironment (EE) on hippocampal-related behavioral impairmentinduced by prenatal restraint stress.􀀲regnant female 􀀤alb/cmice 􀁙ere individually restrained 􀀔 hours/day, since the 􀀓􀀖thday of gestation until delivery. 􀀱ffspring 􀁙ere initially divided intodifferent groups􀀜 prenatal stress 􀀊􀀲S􀀋, no prenatal stress 􀀊C􀀋, inturn each group 􀁙as treated 􀁙ith EE. The offspring 􀁙ere testing􀀛􀀒 days after birth.􀀤alb/c subjected to 􀀲S sho􀁙ed impairment inhabituation memory to an 􀀱pen Field Test, and this 􀁙as revertedmeaningfully by the EE 􀀊p􀀞􀀒.􀀒􀀗􀀋. 􀀫n addition, an increase in anxietybehaviors 􀁙as observed in 􀀲S mice 􀀊p􀀞􀀒.􀀒􀀗􀀋, 􀁙hich 􀁙as revertedby EE in the elevated plus maze test. 􀀫n an 􀀱bject in place test,􀀲S performance in the discrimination tas􀁍 􀁙as altered 􀁙hencompared to control mice 􀀊p􀀞􀀒.􀀒􀀗􀀋, and EE could not revert thisdisability. 􀀯oreover, a significant increase 􀀊p􀀞􀀒.􀀒􀀗􀀋in glucocorticoidreceptors 􀀊􀀩􀀴􀀋 􀁙as found in both the hippocampus and lymphoidcells of 􀀲S mice, but 􀁙as reverted by EE, up to control levels.Corticosterone plasma levels 􀁙ere increased in acute stress miceand in 􀀲S􀀍acute stress mice, the first sho􀁙ing higher levels. EEhas proven successful in reversing this effect in the 􀀲S group. 􀀫nconclusion, these results support the idea that PS induces changesin the H􀀲A axis, 􀁙ith a subsequent altered response to stressexposure in the adult life. The behavioral alteration provo􀁍ed by􀀲S could be related to higher levels of 􀀩􀀴 in the hippocampusand lymphatic ganglia. These changes 􀁙ere partially revertedby EE. Finally, it is important to note that lymphocytes could beperipheral mar􀁍ers of H􀀲A alteration induced by 􀀲S. Funding 􀀲􀀫􀀲􀀓􀀓􀀔􀀔􀀒􀀓􀀕􀀒􀀓 􀀒􀀒􀀖􀀒􀀛 and 􀀷􀀤ACyT 􀀔􀀒􀀒􀀔􀀒􀀓􀀕􀀒􀀓􀀒􀀒􀀓􀀕􀀓.