Evaluation of enzyme carriers as biocatalysts for the conversion of emulsified rhamnolipids

IVANA MAGARIO; ANKE NEUMANN; OLIVER VIELHAUER; CHRISTOPH SYLDATK; RUDOLF HAUSMANN

BIOCATALYSIS AND BIOTRANSFORMATION

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2009 vol. 27 p. 237 - 245

1024-2422

Different carriers were evaluated for alpha-L-rhamnosidase immobilization with respect to activity yield, activity loading and operational stability for the conversion of emulsified di-rhamnolipid to mono-rhamnolipid and L-rhamnose. The highest specific carrier activity of 88 U g-1 with activity yield of 36% was obtained with micro non-porous carriers. Carrier activities of porous-type carriers were much lower due to strong substrate diffusional limitations. Furthermore, the effect of protein and activating-reagent concentration on immobilized enzyme activities on micro non-porous carriers wasinvestigated by a response surface methodology. Immobilized enzyme activities decreased gradually with protein loading whereas the activating-reagent concentration hardly affected them.