INVESTIGADORES
MONGIAT lucas Alberto
artículos
Título:
Corticosterone affects the differentiation of a neuronal cerebral cortex-derived cell line through modulation of the nicotinic acetylcholine receptor.
Autor/es:
BAIER CJ; FRANCO DL; GALLEGOS CE; MONGIAT LA; DIONISIO L; BOUZAT C; CAVIEDES P; BARRANTES FJ
Revista:
NEUROSCIENCE
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2014 p. 1 - 14
ISSN:
0306-4522
Resumen:
Chronic exposure to stress hormones has an impact on brain structures
relevant to cognition. Nicotinic acetylcholine receptors (AChRs) are
involved in numerous cognitive processes including learning and memory
formation. In order to better understand the molecular mechanisms of
chronic stress-triggered mental disease, the effect of corticosterone
(CORT) on the biology of AChRs was studied in the neuronal cell line
CNh. We found that chronic treatment with CORT reduced the expression
levels of the α7-type neuronal AChR and, to a lesser extent, of α4-AChR.
CORT also delayed the acquisition of the mature cell phenotype in CNh
cells. Chronic nicotine treatment affected the differentiation of CNh
cells and exerted a synergistic effect with CORT, suggesting that AChR
could participate in signaling pathways that control the cell cycle.
Overexpression of α7-AChR-GFP abolished the CORT effects on the cell
cycle and the specific α7-AChR inhibitor, methyllycaconitine, mimicked
the proliferative action exerted by CORT. Whole-cell voltage-clamp
recordings showed a significant decrease in nicotine-evoked currents in
CORT-treated cells. Taken together, these observations indicate that
AChRs, and the α7-AChR in particular, could act as modulators of the
differentiation of CNh cells and that CORT could impair the acquisition
of a mature phenotype by affecting the function of this AChR subtype.