INVESTIGADORES
MASCOTTI Maria laura
artículos
Título:
Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
Autor/es:
NICOLL, CALLUM R.; MASSARI, MARTA; FRAAIJE, MARCO W.; MASCOTTI, MARIA LAURA; MATTEVI, ANDREA
Revista:
CURRENT OPINION IN STRUCTURAL BIOLOGY
Editorial:
CURRENT BIOLOGY LTD
Referencias:
Año: 2023 vol. 82
ISSN:
0959-440X
Resumen:
Ancestral sequence reconstruction (ASR) provides insight intothe changes within a protein sequence across evolution. Morespecifically, it can illustrate how specific amino acid changesgive rise to different phenotypes within a protein family. Overthe last few decades it has established itself as a powerfultechnique for revealing molecular common denominators thatgovern enzyme function. Here, we describe the strength ofASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlightingbiotechnological applications the methodology can provide.
