INVESTIGADORES
DAIN Liliana Beatriz
artículos
Título:
Biochemical And Molecular Studies On The Proacrosin/Acrosin System In Patients With Unexplained Infertility
Autor/es:
MARÍ S.; RAWE V.; BIANCOTTI J.C.; CHARREAU E.H.; BRUGO OLMEDO S.; DAIN L.; VAZQUEZ-LEVIN M.
Revista:
FERTILITY AND STERILITY
Editorial:
Elsevier Inc. Eds.
Referencias:
Año: 2003 vol. 79 p. 1676 - 1679
ISSN:
0015-0282
Resumen:
In summary, a relationship was found between a low acrosin
activity and a reduced fertilization rate; and the incidence of abnormal
acrosin activity in male patients with unexplained infertility
was of 19%. The diminished activity apparently was not the result
of a decrease in proacrosin/acrosin acrosomal levels, but rather of
alterations in the proenzyme activation. By using PCR-SSCP analysis,
no changes were detected in the nucleotide sequence around
the active site of the samples studied. Nucleotide sequence analysis
on the sequence encoding human proacrosin, as well as the 5UTR
region in genomic DNA from these patients, may reveal alterations
that could explain abnormal protein function.
