INVESTIGADORES
JURI AYUB Maximiliano
artículos
Título:
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
Autor/es:
CARRILLO, ANGELES; MANZUR, MARÍA JIMENA; JURI AYUB, MAXIMILIANO
Revista:
International Journal of Immunogenetics
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2024
ISSN:
1744-3121
Resumen:
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.
