INVESTIGADORES
PASQUINI juana Maria
artículos
Título:
Optimizing culture medium composition to improve oligodendrocyte progenitor cell yields in vitro from subventricular zone derived neural progenitor cell neurospheres
Autor/es:
FRANCO, PG; PASQUINI, JM; SILVESTROFF L
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2015 vol. 10
ISSN:
1932-6203
Resumen:
Neural Stem and Progenitor Cells (NSC/NPC) are gathering tangible
recognition for their uses in cell therapy and cell replacement
therapies for human disease, as well as a model system to continue
research on overall neural developmental processes in vitro. The
Subventricular Zone is one of the largest NSC/NPC niches in the
developing mammalian Central Nervous System, and persists through to
adulthood. Oligodendrocyte progenitor cell (OPC) enriched cultures are
usefull tools for in vitro studies as well as for cell replacement
therapies for treating demyelination diseases. We used Subventricular
Zone-derived NSC/NPC primary cultures from newborn mice and compared the
effects of different growth factor combinations on cell proliferation
and OPC yield. The Platelet Derived Growth Factor-AA and BB homodimers
had a positive and significant impact on OPC generation. Furthermore,
heparin addition to the culture media contributed to further increase
overall culture yields. The OPC generated by this protocol were able to
mature into Myelin Basic Protein-expressing cells and to interact with
neurons in an in vitro co-culture system. As a whole, we describe an
optimized in vitro method for increasing OPC.