INVESTIGADORES
MUSCHIETTI jorge Prometeo
congresos y reuniones científicas
Título:
DIFFERENTIAL PROTEOME ANALYSIS CHARACTERIZATION OF ARABIDOPSIS HETEROTRIMERIC Gα SUBUNIT MUTANT
Autor/es:
LISI, CHRISTIAN; FOX, ROMINA; MUSCHIETTI, JORGE; MAZZELLA, AGUSTINA
Lugar:
Ciudad de Tucumán, Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2009
Institución organizadora:
SAIB
Resumen:
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In higher plants many classes of biotic and abiotic signals act through
specific transduction mechanisms to coordinate plant development.
Heterotrimeric G protein signaling is one of the most conserved mechanisms in
eukaryotes. Arabidopsis thaliana
possess only one gene encoding a unique α subunit protein: AtGPA1. The evidence revealed that GPA1 is a central molecule in
transmitting hormone, light and other signals to different effectors molecules.
GPA1 has been described as a positive modulator of cell proliferation and gpa1 mutant leaves display a particular
morphology. GPA1 mediated functions have been well described however only a few
downstream effectors have been identified. Nowadays, proteomic is a powerful technique defining proteins
that change in abundance, form, location and can be used to identify proteins
involved in specific developmental or environmental changes. Here, we obtained
the proteome profile of wild type and gpa1
mutant leaves with the proposed to find differentially expressed proteins. Two
dimensional gel electrophoresis revealed seven protein spots that showed
statistically significant changes (p< 0.05) between the two phenotypes. Five
proteins were more abundant in wild type plants and only two proteins were more
abundant in gpa1 mutants. Spot
excision, trypsin digestion and peptide mass sequencing is being conducting to
obtain protein identities.