INVESTIGADORES
MUSCHIETTI jorge Prometeo
congresos y reuniones científicas
Título:
Arabidopsis thaliana pollen TIPs involved in pollen tube elongation
Autor/es:
SOTO, GABRIELA; FOX, ROMINA; ALLEVA, KARINA; MAZZELLA, AGUSTINA; AMODEO, GABRIELA; MUSCHIETTI, JORGE
Lugar:
Ciudad de Tucumán, Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2009
Institución organizadora:
SAIB
Resumen:
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Reproduction in flowering plants includes processes, like pollen
germination and pollen tube elongation, where water and/ or solutes movements
must be finely regulated. These regulations suggest the participation of
aquaporins mediating water and solutes transport. AtTIP5;1 and AtTIP1;3 are the
only aquaporins that are selectively and highly expressed in Arabidopsis thaliana mature pollen.
In a heterologous system (Xenopus oocytes), both TIPs showed urea and
water transport but neither glycerol nor boric acid (Soto, FEBS Lett. 2008,
582). We found that tip1;3 and tip5;1 homozygous mutant plants showed
significantly reduced pollen tube length under nitrogen starvation. This data
suggests that AtTIP1;3 and AtTIP5;1 are involved in nitrogen metabolism during
pollen tube elongation.
To understand the specific role of both TIPs, we generated transgenic
plants that overexpress GFP-AtTIP5;1 and GFP-AtTIP1;3 fusion proteins studying
their subcelular localization.
