INVESTIGADORES
MUSCHIETTI jorge Prometeo
artículos
Título:
Kinase Partner Protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
Autor/es:
KAOTHIEN, P.; WENGIER, D.; COTTER, R.; KELLEY, D.; JORGE PROMETEO MUSCHIETTI; MCCORMICK, S.
Revista:
PLANT JOURNAL
Referencias:
Año: 2005 vol. 42 p. 492 - 503
ISSN:
0960-7412
Resumen:
The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly
suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK
signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen
cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both
LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation
assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing
KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants
over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are
found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and
potentially play roles in signaling pathways in other tissues.