INVESTIGADORES
MONTSERRAT Javier Marcelo
artículos
Título:
Confined Gold Nanoparticles Enhance the Detection of Small Molecules in Label Free Impedance Aptasensors.
Autor/es:
ANA PEINETI; HELENA CERETTI; MARTÍN MIZRAHI; GRACIELA A. GONZÁLEZ; SILVANA RAMÍREZ; JAVIER MONTSERRAT; FERNANDO BATTAGLINI
Revista:
Nanoscale
Editorial:
Royal Society of Chemistry
Referencias:
Año: 2015 vol. 7 p. 7763 - 7769
ISSN:
2040-3372
Resumen:
A controlled
architecture of nanoelectrodes, with a similar size to small molecule-binding
aptamers, is synthetized inside nanoporous alumina. Gold nanoparticles are
electrogenerated in the alumina cavities with a controlled size (2 nm), showing
a fast electron transfer process toward ferrrocyanide. These caped-free
nanoparticles are easily modified with a thiol-containing aptamer for the label-free
detection of adenosine monophosphate by electrochemical impedance spectroscopy.
Our results show that the use of a limited electrical conducting surface inside
an insulating environment can be very sensitive to conformational changes,
introducing a new approach to the detection of small molecules exemplified here
by the direct and selective detection of adenosine monophosphate in the
nanomolar scale.