Optimal nutrition murine model for the study of non-transmissible chronic diseases: a preliminary study

COLUSSI, NARELLA; ROMERO, PATRICIA; TODARO, JUAN SANTIAGO; OLEA, GABRIELA; RODRÍGUEZ, JUAN PABLO; AGUIRRE, MARÍA VICTORIA

Mar del Plata

Congreso; LI Reunión de Anual de la Asociación Argentina de Farmacología Experimental (SAFE); 2019

Abstract/Resumen: The development of animal models of optimal nutrition, understood as one that acts as a protective factor against chronic non communicable diseases (NCDs) is an area of interest in medical sciences. The general objective of the work was to evaluate the nutritional adequacy of experimental diets based on natural ingredients rich in linolenic acid w3 (ALA; C18:3) in experimental mice through multiparametric determinations. Thirty male mice of the Balb-c strain (28 days) from the Bioterium of the Faculty of Medicine-UNNE (CICUAL MED-UNNE Res protocol No. 02/17) were used. Mice were distributed randomly in 3 lots (10 animals each) provided with water and food ad libitum and were fed for 70 days: LOT 1 CONTROL= commercial balanced diet, LOT 2= diet B with crushed chia seeds (Hispanic sage) and LOT 3= diet C with crushed flax seeds (Linnum usitatissimum L). Growth indicators, biochemical parameters, histological and endogenous bioconversion of ALA were determined. The data were processed with the Prism 6.0 software. Weights did not change significantly among lots. The biochemical levels of glucose, cholesterol and triglycerides were lower in the experimental lots compared to control. Histological studies with H / E and PAS stains did not reveal apparent damage to the tissues in both experimental groups. Diet B fed mice showed bioconversion of ALA in 6.4 ± 0.32 % of eicosapentanoic acid (EPA, C 22: 5) and 22.7 ± 1.13 % of docosahexanoic acid (DHA, C 22: 6) in brains. Mice fed with diet C showed no bioconversion in EPA and 114.30 ± 5.7 % in DHA. In conclusion, diets B and C demonstrated lipid lowering and hypoglycemic properties, induced optimal nutrition without causing histopathological alterations. Diet C formulated with crushed flax seed caused the greatest bioconversion in brains of ALA in DHA. This study might contribute to obtain murine experimental models based in dietary conditioning for the study of NCDs.