Cross-reactivity between Philodrvas patagoniensis venom and tetravalent antivenom to Bothrops species

GAY CLAUDIA CAROLINA; MARUÑAK SILVANA; RODRIGUEZ JUAN PABLO; PEICHOTO MARIA ELISA; LEIVA LAURA; ACOSTA OFELIA

San Luis Argentina

Congreso; XXXV Reunión Anual de la Sociedad Argentina de Farmacología Experimental; 2003

SAFE

The aim of the present work was to detect if the antibothropic serum is able to neutralize Philodryas patagoniensis venom. This colubrid snake inhabits the northeast region of Argentine and it is considered as not poisonous, but really it has caused serious lesions as described by the literature. According to this, previous studies have demonstrated that its venom exhibits a high hemorrhagic activity. Oucherlony double diffusion showed two immunoprecipitin lines when de P. patagoniensis venom was tested with tetravalent antivenom to Bothrops species. Hemorrhagic activity of the colubrid snake venom was assayed in mice by Kondo method. This activity was neutralized when venom was pre-incubated with the antiserum. Results showed that there is a cross-reaction between antigens present in P. patagoniensis venom and antibothropic serum. It has relevance since both, antigens and antibodies, belong to different species. This preliminary studies let us predict the potential use of the antibothropic serum, which is available in hospitals, for the treatment of Philodryas patagoniensis intoxication.