Cross reaction between bothropic venoms and IgG anti-crotalic phospholipase A2

RODRÍGUEZ, JUAN PABLO; DE MARZI MAURICIO; MARUÑAK SILVANA; TEIBLER PAMELA; ACOSTA, OFELIA; LEIVA LAURA CRISTINA; MALCHIOIDI EMILIO LUIS

BS. AS.

Congreso; XXXIX Reunión annual de la Sociedad Argentina de Farmacología Experimental; 2007

We examined the ability of IgG antibodies obtained in rabbits against PLA2, from Crotalus durissus terrificus venom to cross react with Bothrops venoms (Bothrops alternatus, Bothrops neuwiedii and Bothrops jararacussú) species that inhabit in the noreastern of Argentina. ELISA and Immunoblotting were performed between bothropic venoms and a desired amount of IgG anti-crotalic PLA2. Bothropic venoms preincubated with IgG anti crotalic PLA2 were i.m. inoculated in mice and then CK level were determinate in sera. Similar mixtures were assayed in red-blood lecithin agar plaque to evaluate the neutralization of indirect hemolytic activity exhibited by these whole venoms. Immunochemical assays showed that IgG anti-PLA2 recognized antigens of Bothropic venoms, and demonstrated the cross reactivity among them. However, biological activity neutralization tests reveled that the amount of IgG anti-PLA2 that efficiently neutralize PLA2 activity of C.d.t. venom was insufficient to neutralize biological activities of Bothropic venoms These results reveal that cross reaction occurs, but large amounts of proteins are needed to neutralize bothropic PLA2s. Since that those proteins are responsible for adverse reactions, the use of these antibodies alone as antivenoms or to enrich commercial antivenoms is not recommendable.