SOY-BASED DIET MODIFIES THE INFILTRATION PROCESS IN CADMIUM INTOXICATED LUNGS

BOLDRINI G; MARTIN MOLINERO G; SALINAS ELOY; CIMINARI E ; BIAGGIO VS ; ALVAREZ SM ; GOMEZ NN

Mar del Plata

Congreso; Sociedad Argentina de Investigacion Clinica; 2019

Sociedad Argentina de Investigacion Clinica

Cadmium (Cd) is a toxicmetal and also is an important environmental contaminant. Previously weobserved by optical microscopy that Cd induces lung infiltration, so that westudied Cd intoxication and the parallels effects on hematological parametersand bronchoalveolar lavages (BAL) under different diets. 4 lots of femaleWistar rats were used: 2 lots received casein (Cas) and 2 lots received soybean(Soy) as protein sources. Within each group: 1 lot received regular water(control-Co) and the other 15ppm of Cd in drinking water for 60 days. Bloodsamples were collected and they were analyzed using a hemocytometer ADVIA 360(SIEMENS) and autoanalyzer A15 (BIOSYSTEMS). BAL was performed, cells werecounted using a Neubauer?s chamber and LDH activity was measured. Total RNA was isolated from lungs using Trizol andcDNA was obtained: VCAM-1 and Nrf2 were determined by PCR, using S28 ascontrol. ANOVAtest was used for statistical analysis.White blood cells decreased in both intoxicated groups (p<0.05). No significantdifferences among the percentages of lymphocytes (LYM), monocytes (MID) andgranulocytes (GRA) were found. Nevertheless, MID showed a trend to increase inCasCd group and GRA showed the same trend in SoCd group. Hematocrit was lowerin Soy vs Cas groups (p<0.05). While total red blood cells were decreased inSoCo vs CasCo (p<0.01), MCV was diminished in SoCd vs CasCd (p<0.01). RDWdecreased in SoCo vs CasCo (p<0.05). Hemoglobin decreased in SoCo vs CasCo(p<0.05). MCHC was significantly augmented in SoCd vs CasCd (p<0.05). Uricacid was increased in CasCd vs CasCo (p<0.05) and decreased in SoCd vs CasCd(p<0.01).On the other hand, LYM increased in BAL of both Cd-intoxicated groupsand GRA augmented in SoCd. LDH activity increased in CasCd vs CasCo (p<0.001)and decreased in SoCd vs CasCd (p<0.0001). Concerning to lung tissue, VCAM-1expression increased in SoCd vs SoCo (p<0.05) and showed the same trend inCasCd vs CasCo, while Nrf-2 expression augmented only in SoCd vs SoCo(p<0,01) and vs CasCd (p<0.01).Based on these results we concluded that drinking water Cd inducesoxidative stress on lungs and modifies hematological parameters. Uric acidlevels showed a systemic oxidative stress in Cas-fed animals in presence of Cd.On the other hand, Nrf-2 expression showed an oxidative stress response only inSoy-fed animals. Changes on hematological parameters, especially WBC, could berelated with changes in BAL cells population through modifications on VCAM-1expression.