INVESTIGADORES
BIAGGIO Veronica Silvina
congresos y reuniones científicas
Título:
LA DEFICIENCIA DIETARIA DE ZINC INDUCE CAMBIOS EN EL METABOLISMO LIPIDICO DEL PULMON
Autor/es:
ROZZEN,E; BIAGGIO,VS; ALVAREZ,SM; GIMÉNEZ,MS; GÓMEZ,NN
Lugar:
Merlo - San Luis
Reunión:
Otro; XXII REUNION CIENTÍFICA ANUAL SOCIEDAD DE BIOLOGÍA DE CUYO . II REUNION ANUAL SAMIC.; 2004
Institución organizadora:
Universidad Nacional de San Luis
Resumen:
ABSTRACT
Zinc deficiency is a widespread problem. Zn essential roles in many
aspects of the metabolism (Vallee and Falchuk, 1993), includes the
activity of more than 300 enzymes, the structure of many proteins,
and control of genetic expression. Zn chronic deficiency modified
lipid parameters, especially phospholipids and cholesterol (Gómez
et al., 2002). The present study was conducted to investigate the
effects of Zn deficiency on the expression of lipidic enzymes such
as ACC, LPL, HMG-CoA R, GPAT and SREBP2 in rat lung. Wistar
male rats (200 ± 20 g) were fed with AIN-93 diet (zinc-deficient,
ZD) or with 30 mg Zn/ kg (Control, Co) during 2 months. Total
RNA was isolated from lung by using TRIzol. 1 mg of RNA was
transcribed to cDNA at 42°C using random hexamers as primers
and RT-MMLV (Moloney Murine Leukemia Virus). Aliquots of 2
mL of cDNA were used in the amplifications by PCR using specific
primers. Beta actin was used as an internal control. We detected a
significant increase in ACC (p < 0.01), LPL (p < 0.005) and SREBP2
(p < 0.01). HMG-CoA R (p < 0.005) decreased significantly, while
GPAT did not change. From the results of this study, it can be
concluded that Zn deficiency produces numerous alterations on
lipids metabolism in lung.